Derivative matrix isopotential synchronous fluorescence spectroscopy for the direct determination of 1-hydroxypyrene as a urinary biomarker of exposure to polycyclic aromatic hydrocarbons.

Solid Phase Extraction for 1-Hydroxypyrene as a Biomarker of Occupational Exposure to PAHs Prior to High Performance Liquid Chromatography

Urinary 1-hydroxypyrene is frequently used as a major metabolite and biological indicator of the overall exposure to polycyclic aromatic hydrocarbons. In this study, solid phase extraction was appropriately conditioned with regard to sample pH, sample concentration, loading flow rate, elution solvent, washing solvent, sample volume and sorbent mass. Octadecyl silica cartridge (C<su...

1-Hydroxypyrene as a biomarker of PAH exposure in the marine polychaete Nereis diversicolor.

The possibility of using the pyrene metabolite 1-hydroxypyrene as a biomarker of polycyclic aromatic hydrocarbons (PAHs) exposure was investigated by exposure of the marine polychaete Nereis diversicolor to several PAHs in the laboratory. Animals were exposed to pyrene alone and to five different PAHs - phenanthrene, anthracene, pyrene, benzo[a]pyrene and benzo[k]flouranthene. After five days o...

Implementation, Validation and Application of a Method of Evaluation of Urinary 1-hydroxypyrene as an Indicator of Human Exposure to Polycyclic Aromatic Hydrocarbons in Rio De Janeiro State, Brazil

− Epidemiological studies have shown that the exposure to polycyclic aromatic hydrocarbons (PAHs) increases the risk of several kinds of human cancers. In this way the use of biomarkers to evaluate PAH exposure is of interest. Urinary 1-hydroxypyrene (1-OHPy) that is the major metabolite of pyrene has been considered as a suitable biomarker of exposure to PAHs as a group, but there are few data...

1-Hydroxypyrene as a biomarker for fish exposure to polycyclic aromatic hydrocarbons.

Exposure of iron foundry workers to polycyclic aromatic hydrocarbons: benzo(a)pyrene-albumin adducts and 1-hydroxypyrene as biomarkers for exposure.

Exposure to polycyclic aromatic hydrocarbons (PAHs) in foundry workers has been evaluated by determination of benzo(a)pyrene-serum albumin adducts and urinary 1-hydroxypyrene. Benzo(a)pyrene binding to albumin and 1-hydroxypyrene were quantitatively measured by enzyme linked immunosorbent assay (ELISA) and reverse phase high performance liquid chromatography (HPLC), respectively. 70 male foundr...